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Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 at 10 ug/mL for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Flow Cytometry

Flow cytometry analysis of PBMC’s were stained with unimmunized rabbit IgG antibody (black line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer.

Entrez GeneID

3678

Protein Accession#

P08648

Gene Name

ITGA5

Gene Alias

CD49e, FNRA, VLA5A

Gene Description

integrin, alpha 5 (fibronectin receptor, alpha polypeptide)

Omim ID

135620

Gene Ontology

Hyperlink

Gene Summary

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq

Other Designations

fibronectin receptor, alpha subunit|integrin alpha 5|very late activation protein 5, alpha subunit