ITGA5 recombinant monoclonal antibody, clone R02-3G4
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562 and Lane 2: 3T3 lysates with ITGA5 recombinant monoclonal antibody, clone R02-3G4 (Cat # RAB02170).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with ITGA5 recombinant monoclonal antibody, clone R02-3G4 (Cat # RAB02170). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ITGA5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ITGA5.
Theoretical MW (kDa)
Calculated MW: 115 k
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562 and Lane 2: 3T3 lysates with ITGA5 recombinant monoclonal antibody, clone R02-3G4 (Cat # RAB02170).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with ITGA5 recombinant monoclonal antibody, clone R02-3G4 (Cat # RAB02170). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — ITGA5
Entrez GeneID
3678Protein Accession#
P08648Gene Name
ITGA5
Gene Alias
CD49e, FNRA, VLA5A
Gene Description
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Omim ID
135620Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq
Other Designations
fibronectin receptor, alpha subunit|integrin alpha 5|very late activation protein 5, alpha subunit
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Interactome
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Pathway
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Disease
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