MAP4K5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with MAP4K5 polyclonal antibody (Cat # PAB30532)
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with MAP4K5 polyclonal antibody (Cat # PAB30532) shows moderate positivity in a subset of cells in seminiferus ducts.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with MAP4K5 polyclonal antibody (Cat # PAB30532) shows positivity in plasma membrane and cytoplasm. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human MAP4K5.
Immunogen
Recombinant protein corresponding to human MAP4K5.
Sequence
YPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLPRKKDKRDFPKPA
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with MAP4K5 polyclonal antibody (Cat # PAB30532)
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with MAP4K5 polyclonal antibody (Cat # PAB30532) shows moderate positivity in a subset of cells in seminiferus ducts.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with MAP4K5 polyclonal antibody (Cat # PAB30532) shows positivity in plasma membrane and cytoplasm. Antibody staining is shown in green. -
Gene Info — MAP4K5
Entrez GeneID
11183Gene Name
MAP4K5
Gene Alias
GCKR, KHS, KHS1, MAPKKKK5
Gene Description
mitogen-activated protein kinase kinase kinase kinase 5
Omim ID
604923Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq
Other Designations
germinal center kinase-related|kinase homologous to SPS1/STE20
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Interactome
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Disease
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