Products

Western Blot (Transfected lysate)

Western Blot analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody (M01), clone 3H3.

Lane 1: MAP4K5 transfected lysate(95.04 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged MAP4K5 is approximately 0.03ng/ml as a capture antibody.

ELISA

RNAi Knockdown (Antibody validated)

Western blot analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi ( Cat # H00011183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP4K5 monoclonal antibody (M01) clone 3H3 (Cat # H00011183-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Entrez GeneID

11183

GeneBank Accession#

BC036013

Protein Accession#

AAH36013

Gene Name

MAP4K5

Gene Alias

GCKR, KHS, KHS1, MAPKKKK5

Gene Description

mitogen-activated protein kinase kinase kinase kinase 5

Omim ID

604923

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq

Other Designations

germinal center kinase-related|kinase homologous to SPS1/STE20