VCL polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with VCL polyclonal antibody (Cat # PAB30250) at 1:100 - 1:250 dilution
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Lane 3: Human cell line A-431
Lane 4: Human liver tissue
Lane 5: Human tonsil tissue
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skeletal muscle with VCL polyclonal antibody (Cat # PAB30250) shows sstrong cytoplasmic positivity in smooth muscle cells at 1:50 - 1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human VCL.
Immunogen
Recombinant protein corresponding to amino acids of human VCL.
Sequence
KMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREI
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis with VCL polyclonal antibody (Cat # PAB30250) at 1:100 - 1:250 dilution
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Lane 3: Human cell line A-431
Lane 4: Human liver tissue
Lane 5: Human tonsil tissueImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skeletal muscle with VCL polyclonal antibody (Cat # PAB30250) shows sstrong cytoplasmic positivity in smooth muscle cells at 1:50 - 1:200 dilution. -
Gene Info — VCL
Entrez GeneID
7414Protein Accession#
P18206Gene Name
VCL
Gene Alias
CMD1W, MVCL
Gene Description
vinculin
Gene Ontology
HyperlinkGene Summary
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000019861|OTTHUMP00000043885|metavinculin
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Interactome
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Pathway
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Disease
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