VCL recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with VCL recombinant monoclonal antibody (Cat # RAB02557).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using VCL recombinant monoclonal antibody (Cat # RAB02557). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.18). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human VCL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human VCL.
Theoretical MW (kDa)
124
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Vinculin protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with VCL recombinant monoclonal antibody (Cat # RAB02557).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using VCL recombinant monoclonal antibody (Cat # RAB02557). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.18). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Gene Info — VCL
Entrez GeneID
7414Protein Accession#
P18206Gene Name
VCL
Gene Alias
CMD1W, MVCL
Gene Description
vinculin
Gene Ontology
HyperlinkGene Summary
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000019861|OTTHUMP00000043885|metavinculin
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Interactome
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Pathway
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Disease
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