VCL polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of VCL.
Immunogen
A synthetic peptide corresponding to residues surrounding tyrosine 821 of human VCL.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total VCL protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with forskolin (40nM, 30 mins), using VCL polyclonal antibody (Cat # PAB17308).
Peptide "+" means "with peptide blocking". -
Gene Info — VCL
Entrez GeneID
7414Protein Accession#
Q64727Gene Name
VCL
Gene Alias
CMD1W, MVCL
Gene Description
vinculin
Gene Ontology
HyperlinkGene Summary
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000019861|OTTHUMP00000043885|metavinculin
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Interactome
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Pathway
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Disease
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Publication Reference
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Vimentin-dependent utilization of LDL-cholesterol in human adrenal tumor cells is not associated with the level of expression of apoE, sterol carrier protein-2, or caveolin.
Holwell TA, Schweitzer SC, Reyland ME, Evans RM.
Journal of Lipid Research 1999 Aug; 40(8):1440.
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Disrupted glial fibrillary acidic protein network in astrocytes from vimentin knockout mice.
Galou M, Colucci-Guyon E, Ensergueix D, Ridet JL, Gimenez y Ribotta M, Privat A, Babinet C, Dupouey P.
Journal of Cellular Biology 1996 May; 133(4):853.
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Vimentin expression is differentially regulated by IL-2 and IL-4 in murine T cells.
Hornbeck PV, Garrels JI, Capetanaki Y, Heimer S.
The Journal of Immunology 1993 Oct; 151(8):4013.
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Vimentin-dependent utilization of LDL-cholesterol in human adrenal tumor cells is not associated with the level of expression of apoE, sterol carrier protein-2, or caveolin.
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