CLTA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG Lane 3: Human plasma Lane 4: Human liver tissue Lane 5: Human tonsil tissue with CLTA polyclonal antibody (Cat # PAB29433) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with CLTA polyclonal antibody (Cat # PAB29433) shows strong membranous positivity in cells in tubules at 1:2500-1:5000 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with CLTA polyclonal antibody (Cat # PAB29433) at 1-4 ug/mL concentration shows positivity in vesicles.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human CLTA.
Immunogen
Recombinant protein corresponding to human CLTA.
Sequence
NDEAFAILDGGAPGPQPHGEPPGGPDAVDGVMNGEYYQESNGPTDSYAAISQVDRLQSEPE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:2500-1:5000)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG Lane 3: Human plasma Lane 4: Human liver tissue Lane 5: Human tonsil tissue with CLTA polyclonal antibody (Cat # PAB29433) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with CLTA polyclonal antibody (Cat # PAB29433) shows strong membranous positivity in cells in tubules at 1:2500-1:5000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with CLTA polyclonal antibody (Cat # PAB29433) at 1-4 ug/mL concentration shows positivity in vesicles. -
Gene Info — CLTA
Entrez GeneID
1211Gene Name
CLTA
Gene Alias
LCA
Gene Description
clathrin, light chain (Lca)
Omim ID
118960Gene Ontology
HyperlinkGene Summary
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000021365|OTTHUMP00000021366|clathrin, light polypeptide (Lca)|clathrin, light polypeptide A
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Interactome
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Pathway
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Disease
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