CLTA/CLTB polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CLTA/CLTB polyclonal antibody (Cat # PAB11423) (0.1 ug/mL) staining of human frontal cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CLTA/CLTB.
Immunogen
A synthetic peptide corresponding to human CLTA/CLTB.
Sequence
CDFNPKSSKQAKD
Host
Goat
Theoretical MW (kDa)
23.7, 27.1, 25.7, 23
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CLTA/CLTB polyclonal antibody (Cat # PAB11423) (0.1 ug/mL) staining of human frontal cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CLTA
Entrez GeneID
1211Protein Accession#
NP_001824.1 (Gene ID : 1211);NP_009027.1 (Gene ID : 1211);NP_001070145.1 (Gene ID : 1211);NP_001825.1 (Gene ID : 1212);NP_009028.1 (Gene ID : 1212)Gene Name
CLTA
Gene Alias
LCA
Gene Description
clathrin, light chain (Lca)
Omim ID
118960Gene Ontology
HyperlinkGene Summary
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000021365|OTTHUMP00000021366|clathrin, light polypeptide (Lca)|clathrin, light polypeptide A
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Gene Info — CLTB
Entrez GeneID
1212Protein Accession#
NP_001824.1 (Gene ID : 1211);NP_009027.1 (Gene ID : 1211);NP_001070145.1 (Gene ID : 1211);NP_001825.1 (Gene ID : 1212);NP_009028.1 (Gene ID : 1212)Gene Name
CLTB
Gene Alias
LCB
Gene Description
clathrin, light chain (Lcb)
Omim ID
118970Gene Ontology
HyperlinkGene Summary
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
clathrin, light polypeptide|clathrin, light polypeptide (Lcb)
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Interactome
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Pathway
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Disease
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Publication Reference
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Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly.
Poupon V, Girard M, Legendre-Guillemin V, Thomas S, Bourbonniere L, Philie J, Bright NA, McPherson PS.
PNAS 2007 Dec; 105(1):168.
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Clathrin in gastric acid secretory (parietal) cells: biochemical characterization and subcellular localization.
Okamoto CT, Duman JG, Tyagarajan K, McDonald KL, Jeng YY, McKinney J, Forte TM, Forte JG.
American Journal of Physiology. Cell Physiology 2000 Sep; 279(3):C833.
Application：IF, WB-Ti, Pig, Brain, Gastric microsomes, Parietal cells.
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Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly.
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