BCL2 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using BCL2 polyclonal antibody (Cat # PAB2440) for immunoprecipitation (IP) and a different BCL2 antibody for western blot. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.
Flow Cytometry
Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) using BCL2 polyclonal antibody (Cat # PAB2440) in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BCL2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the BH3 domain of human BCL2.
Host
Rabbit
Reactivity
Human
Specificity
BH3 Domain Specific
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
Immunoprecipitation (1:100-500)
Flow cytometry (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using BCL2 polyclonal antibody (Cat # PAB2440) for immunoprecipitation (IP) and a different BCL2 antibody for western blot. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.Immunohistochemistry
Immunoprecipitation
Flow Cytometry
Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) using BCL2 polyclonal antibody (Cat # PAB2440) in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences. -
Gene Info — BCL2
Entrez GeneID
596Protein Accession#
NP_000624;P10415Gene Name
BCL2
Gene Alias
Bcl-2
Gene Description
B-cell CLL/lymphoma 2
Omim ID
151430Gene Ontology
HyperlinkGene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
Other Designations
B-cell lymphoma protein 2|OTTHUMP00000163680
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Interactome
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Pathway
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Disease
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Publication Reference
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Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues.
Liu J, Zhou W, Li SS, Sun Z, Lin B, Lang YY, He JY, Cao X, Yan T, Wang L, Lu J, Han YH, Cao Y, Zhang XK, Zeng JZ.
Cancer Research 2008 Nov; 68(21):8871.
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Protein phosphatase 2A inactivates Bcl2's antiapoptotic function by dephosphorylation and up-regulation of Bcl2-p53 binding.
Deng X, Gao F, May WS.
Blood 2008 Oct; 113(2):422.
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Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues.
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