MAPK8/MAPK9/MAPK10 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAPK8/MAPK9/MAPK10.
Immunogen
A synthetic peptide corresponding to human MAPK8/MAPK9/MAPK10.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to MAPK8/MAPK9/MAPK10.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:100-1:500)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with UV (5 mins), using MAPK8/MAPK9/MAPK10 polyclonal antibody (Cat # PAB18183).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using MAPK8/MAPK9/MAPK10 polyclonal antibody (Cat # PAB18183).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
-
Gene Info — MAPK8
Entrez GeneID
5599Gene Name
MAPK8
Gene Alias
JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1
Gene Description
mitogen-activated protein kinase 8
Omim ID
601158Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
JNK1 alpha protein kinase|JNK1 beta protein kinase|JUN N-terminal kinase|OTTHUMP00000019552|OTTHUMP00000019555|OTTHUMP00000019556|OTTHUMP00000019558|c-Jun N-terminal kinase 1|mitogen-activated protein kinase 8 isoform JNK1 alpha1|mitogen-activated protein
-
Gene Info — MAPK9
Entrez GeneID
5601Gene Name
MAPK9
Gene Alias
JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, p54a, p54aSAPK
Gene Description
mitogen-activated protein kinase 9
Omim ID
602896Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
Jun kinase|MAP kinase 9|c-Jun N-terminal kinase 2|c-Jun kinase 2|mitogen-activated protein kinase 9 isoform JNK2 alpha2|stress-activated protein kinase JNK2
-
Gene Info — MAPK10
Entrez GeneID
5602Gene Name
MAPK10
Gene Alias
FLJ12099, FLJ33785, JNK3, JNK3A, MGC50974, PRKM10, p493F12, p54bSAPK
Gene Description
mitogen-activated protein kinase 10
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
JNK3 alpha protein kinase|MAP kinase|OTTHUMP00000161180|OTTHUMP00000161182|OTTHUMP00000161183|c-Jun N-terminal kinase 3|c-Jun kinase 3|stress activated protein kinase JNK3|stress activated protein kinase beta
-
Interactome
-
Pathway
- Adipocytokine signaling pathway
- Adipocytokine signaling pathway
- Adipocytokine signaling pathway
- Colorectal cancer
- Colorectal cancer
- Colorectal cancer
- Epithelial cell signaling in Helicobacter pylori infection
- Epithelial cell signaling in Helicobacter pylori infection
- Epithelial cell signaling in Helicobacter pylori infection
+ View More Disease
-
Disease
-
Publication Reference
-
Genetic inhibition or activation of JNK1/2 protects the myocardium from ischemia-reperfusion-induced cell death in vivo.
Kaiser RA, Liang Q, Bueno O, Huang Y, Lackey T, Klevitsky R, Hewett TE, Molkentin JD.
The Journal of Biological Chemistry 2005 Sep; 280(38):32602.
-
c-Jun N-terminal protein kinase (JNK) 2/3 is specifically activated by stress, mediating c-Jun activation, in the presence of constitutive JNK1 activity in cerebellar neurons.
Coffey ET, Smiciene G, Hongisto V, Cao J, Brecht S, Herdegen T, Courtney MJ.
The Journal of Neuroscience 2002 Jun; 22(11):4335.
Application:IP, WB-Ce, WB-Ti, Monkey, Mouse, COS-7 cells, Neurons.
-
Differential gene expression profiles of Jnk1- and Jnk2-deficient murine fibroblast cells.
Chen N, She QB, Bode AM, Dong Z.
Cancer Research 2002 Mar; 62(5):1300.
-
Genetic inhibition or activation of JNK1/2 protects the myocardium from ischemia-reperfusion-induced cell death in vivo.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com