MAPK8 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:PC12 whole cell lysate Lane3:K562 whole cell lysate with MAPK8 recombinant monoclonal antibody (Cat # RAB02669) at 1:1000 dilution.
Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using MAPK8 recombinant monoclonal antibody (Cat # RAB02669)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against MAPK8.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant MAPK8.
Theoretical MW (kDa)
46, 54
Reactivity
Chicken, Human, Monkey, Mouse, Rat, Zebra fish
Specificity
This antibody detects endogenous levels of JNK1/2/3 proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:PC12 whole cell lysate Lane3:K562 whole cell lysate with MAPK8 recombinant monoclonal antibody (Cat # RAB02669) at 1:1000 dilution.Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using MAPK8 recombinant monoclonal antibody (Cat # RAB02669)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — MAPK8
Entrez GeneID
5599Protein Accession#
P45983Gene Name
MAPK8
Gene Alias
JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1
Gene Description
mitogen-activated protein kinase 8
Omim ID
601158Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
JNK1 alpha protein kinase|JNK1 beta protein kinase|JUN N-terminal kinase|OTTHUMP00000019552|OTTHUMP00000019555|OTTHUMP00000019556|OTTHUMP00000019558|c-Jun N-terminal kinase 1|mitogen-activated protein kinase 8 isoform JNK1 alpha1|mitogen-activated protein
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Interactome
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Pathway
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Disease
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