CRKL polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, using CRKL polyclonal antibody (Cat # PAB18120).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using CRKL polyclonal antibody (Cat # PAB18120).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using CRKL polyclonal antibody (Cat # PAB18120).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CRKL.
Immunogen
A synthetic peptide corresponding to human CRKL.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to CRKL.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, using CRKL polyclonal antibody (Cat # PAB18120).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using CRKL polyclonal antibody (Cat # PAB18120).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using CRKL polyclonal antibody (Cat # PAB18120).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CRKL
Entrez GeneID
1399Protein Accession#
P46109Gene Name
CRKL
Gene Alias
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Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Omim ID
602007Gene Ontology
HyperlinkGene Summary
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic
Other Designations
v-crk avian sarcoma virus CT10 oncogene homolog-like
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Interactome
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Pathway
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Disease
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