MAPK8 (phospho T183/Y185) polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAPK8.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T183/Y185 of human MAPK8.
Sequence
TPY
Host
Rabbit
Theoretical MW (kDa)
49, 55
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes human MAPK8.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from UV treated HEK 293 was immunoblotted by MAPK8 (phospho T183/Y185) polyclonal antibody (Cat # PAB12630) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK8
Entrez GeneID
5599Gene Name
MAPK8
Gene Alias
JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1
Gene Description
mitogen-activated protein kinase 8
Omim ID
601158Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
JNK1 alpha protein kinase|JNK1 beta protein kinase|JUN N-terminal kinase|OTTHUMP00000019552|OTTHUMP00000019555|OTTHUMP00000019556|OTTHUMP00000019558|c-Jun N-terminal kinase 1|mitogen-activated protein kinase 8 isoform JNK1 alpha1|mitogen-activated protein
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Interactomes
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Pathways
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Diseases
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Publication Reference
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DNA damage-dependent translocation of B23 and p19 ARF is regulated by the Jun N-terminal kinase pathway.
Yogev O, Saadon K, Anzi S, Inoue K, Shaulian E.
Cancer Research 2008 Mar; 68(5):1398.
Application:WB-Ce, Mouse, MEF cells.
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DNA damage-dependent translocation of B23 and p19 ARF is regulated by the Jun N-terminal kinase pathway.
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