STAT1 (phospho S727) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S727 of human STAT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of STAT1 (phospho S727) polyclonal antibody (Cat # PAB0613) in mouse brain tissue lysate (35 ug/lane). Phospho-STAT1 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with STAT1 (phospho S727) polyclonal antibody (Cat # PAB0613) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — STAT1
Entrez GeneID
6772Protein Accession#
NP_009330;P42224Gene Name
STAT1
Gene Alias
DKFZp686B04100, ISGF-3, STAT91
Gene Description
signal transducer and activator of transcription 1, 91kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000165047|signal transducer and activator of transcription 1|signal transducer and activator of transcription-1|transcription factor ISGF-3 components p91/p84
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Interactome
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Pathway
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Publication Reference
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A short peptide at the amino terminus of the Sendai virus C protein acts as an independent element that induces STAT1 instability.
Garcin D, Marq JB, Iseni F, Martin S, Kolakofsky D.
Journal of Virology 2004 Aug; 78(16):8799.
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Expression of hepatitis C virus core protein inhibits interferon-induced nuclear import of STATs.
Melen K, Fagerlund R, Nyqvist M, Keskinen P, Julkunen I.
Journal of Medical Virology 2004 Aug; 73(4):536.
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Requirement of histone deacetylase activity for signaling by STAT1.
Klampfer L, Huang J, Swaby LA, Augenlicht L.
The Journal of Biological Chemistry 2004 Jul; 279(29):30358.
Application:WB, Human, Hke-3 cells.
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A short peptide at the amino terminus of the Sendai virus C protein acts as an independent element that induces STAT1 instability.
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