STAT1 (phospho S727) recombinant monoclonal antibody, clone 2H10
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: HepG2 whole cell lysate (not treated) with STAT1 (phospho S727) recombinant monoclonal antibody, clone 2H10 (Cat # RAB04257).
Immunohistochemistry
Immunohistochemical staining of human breast cancer with STAT1 (phospho S727) recombinant monoclonal antibody, clone 2H10 (Cat # RAB04257) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with STAT1 (phospho S727) recombinant monoclonal antibody, clone 2H10 (Cat # RAB04257) (diluated at 1:66). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human STAT1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S727 of human STAT1.
Theoretical MW (kDa)
Calculated MW: 87 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 2: HepG2 whole cell lysate (not treated) with STAT1 (phospho S727) recombinant monoclonal antibody, clone 2H10 (Cat # RAB04257).Immunohistochemistry
Immunohistochemical staining of human breast cancer with STAT1 (phospho S727) recombinant monoclonal antibody, clone 2H10 (Cat # RAB04257) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of HepG2 cells with STAT1 (phospho S727) recombinant monoclonal antibody, clone 2H10 (Cat # RAB04257) (diluated at 1:66). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — STAT1
Entrez GeneID
6772Protein Accession#
P42224Gene Name
STAT1
Gene Alias
DKFZp686B04100, ISGF-3, STAT91
Gene Description
signal transducer and activator of transcription 1, 91kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000165047|signal transducer and activator of transcription 1|signal transducer and activator of transcription-1|transcription factor ISGF-3 components p91/p84
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com