STAT1 recombinant monoclonal antibody, clone Stat1S727-C6
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More Files
- More Functions
Western Blot
Western blot analysis of U937 cell extract untreated or treated with IL-4 using 0.1 ug/mL Phospho-Stat1 (Ser727) antibody Stat1S727-C6 .
Flow Cytometry
Flow cytometric analysis of U937 cells untreated (red) or treated with IFNa+IL4+PV (green) using Phospho-Stat1 (Ser727) (C6) Rabbit mAb Stat1S727-C6 at 0.01 ug/mL , or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells untreated (black) or treated with IFNa+IL4+PV (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human STAT1.
Antibody Species
Rabbit
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human phospho Stat1
Reactivity
Human
Form
Liquid
Purification
Protein A+G
Isotype
Rabbit IgG1k
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
1X PBS, 0.02% Sodium azide, 50% Glycerol, 0.1% BSA
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of U937 cell extract untreated or treated with IL-4 using 0.1 ug/mL Phospho-Stat1 (Ser727) antibody Stat1S727-C6 .Flow Cytometry
Flow cytometric analysis of U937 cells untreated (red) or treated with IFNa+IL4+PV (green) using Phospho-Stat1 (Ser727) (C6) Rabbit mAb Stat1S727-C6 at 0.01 ug/mL , or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells untreated (black) or treated with IFNa+IL4+PV (blue). -
Gene Info — STAT1
Entrez GeneID
6772Protein Accession#
P42224Gene Name
STAT1
Gene Alias
DKFZp686B04100, ISGF-3, STAT91
Gene Description
signal transducer and activator of transcription 1, 91kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000165047|signal transducer and activator of transcription 1|signal transducer and activator of transcription-1|transcription factor ISGF-3 components p91/p84
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Interactome
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Pathway
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Disease
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