FGF21 (Human) Recombinant Protein
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More Files
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Surface Plasmon Resonance
Human FGF21, His Tag immobilized on CM5 Chip can bind Human Human Beta Klotho, His Tag with an affinity constant of 20nM as determined in a SPR assay (Biacore T200).
SEC-HPLC
The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human FGF21 (Q9NSA1-1, His29-Ser209) partial recombinant protein with His tag at N-Terminus expressed in HEK293 cells.
Sequence
His29-Ser209
Host
Human
Theoretical MW (kDa)
20.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The affinity constant of 20nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human FGF21, His Tag immobilized on CM5 Chip can bind Human Human Beta Klotho, His Tag with an affinity constant of 20nM as determined in a SPR assay (Biacore T200). -
Gene Info — FGF21
Entrez GeneID
26291Protein Accession#
Q9NSA1-1Gene Name
FGF21
Gene Alias
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Gene Description
fibroblast growth factor 21
Omim ID
609436Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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