FGF21 (Human) Recombinant Protein
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Specification
Product Description
Human FGF21 recombinant protein expressed in Escherichia coli.
Sequence
HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPAPPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS
Host
Escherichia coli
Theoretical MW (kDa)
19.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 96% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 0.5 ug/mL, measured by thymidine uptake assay using FGF-receptors transfected BaF3 cells, corresponding to a specific activity of > 2.0 x 103 IU/mg in the presence of 5 ug/mL of rMuKlotho-beta and 10 ug/mL of heparin.
Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — FGF21
Entrez GeneID
26291Protein Accession#
Q9NSA1Gene Name
FGF21
Gene Alias
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Gene Description
fibroblast growth factor 21
Omim ID
609436Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq
Other Designations
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Interactome
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