FGF21 (Human) Recombinant Protein
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Specification
Product Description
Human FGF21 (Q9NSA1, 29 a.a. - 209 a.a.) partial recombinant protein with an N-terminal Gly expressed in Escherichia coli.
Sequence
HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS
Host
Escherichia coli
Theoretical MW (kDa)
~ 19.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 0.5 ug/mL, measured by a cell proliferation assay using NIH-3T3 cells in the presence of 1.25 ug/mL mouse Klotho and 10 ug/mL heparin, corresponding to a specific activity of > 2.0 × 103 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — FGF21
Entrez GeneID
26291Protein Accession#
Q9NSA1Gene Name
FGF21
Gene Alias
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Gene Description
fibroblast growth factor 21
Omim ID
609436Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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