Vegfa (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Vegfa partial recombinant protein with His tag in N-terminus expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKCDKPRR
Host
Escherichia coli
Theoretical MW (kDa)
16.3
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 90% as determined by SDS-PAGE.
Activity
ED50 is 0.5-1.5 ng/mL, measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast.
Storage Buffer
Solution containing 20 mM Tris-HCl, pH 8.0, 10% glycerol.
Storage Instruction
Store at 4°C for one weeks and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Vegfa
Entrez GeneID
22339Protein Accession#
Q00731Gene Name
Vegfa
Gene Alias
Vegf, Vegf-a, Vegf120, Vegf164, Vegf188, Vpf
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000017463|OTTMUSP00000017464|OTTMUSP00000022243|vascular permeability factor
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