Vegfa (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Vegfa recombinant protein expressed in Escherichia coli.
Sequence
MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Host
Escherichia coli
Theoretical MW (kDa)
38.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
The biological activity is 5.0 ng/mL, determined by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of 2.0 x 105 IU/mg.
Storage Buffer
Lyophilized from 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Vegfa
Entrez GeneID
22339Protein Accession#
Q00731Gene Name
Vegfa
Gene Alias
Vegf, Vegf-a, Vegf120, Vegf164, Vegf188, Vpf
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000017463|OTTMUSP00000017464|OTTMUSP00000022243|vascular permeability factor
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