VEGFA (Mouse) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Mouse VGFA (Q00731-2, 27 a.a. - 190 a.a.) partial recombinant protein expressed in Pichia pastoris.
Sequence
APTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKKSVRGKGKGQKRKRKKSRFKSWSVHCEPCSERRKHLFVQDPQTCKCSCKN
Host
Yeast
Theoretical MW (kDa)
39
Form
Lyophilized
Preparation Method
Pichia pastoris expression system
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by gel clotting method)
Activity
The ED50 was determined by the dose-dependent stimulation of proliferation of HUVEC cells is < 6.0 ng/ml, corresponding to a specific activity of > 1.7 x 105 units/mg.
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/ml
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Vegfa
Entrez GeneID
22339Protein Accession#
Q00731-2Gene Name
Vegfa
Gene Alias
Vegf, Vegf-a, Vegf120, Vegf164, Vegf188, Vpf
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000017463|OTTMUSP00000017464|OTTMUSP00000022243|vascular permeability factor
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