Vegfa (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Vegfa (Q00731-3, 27 a.a. - 146 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
AAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKCDKPRR
Host
Escherichia coli
Theoretical MW (kDa)
28.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 96% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The ED50 was determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is < 5.0 ng/ml, corresponding to a specific activity of > 2.0 x 105 IU/mg.
Storage Buffer
Lyophilized from sterile distilled Water up to 0.1 - 1.0 mg/ml
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Vegfa
Entrez GeneID
22339Protein Accession#
Q00731-3Gene Name
Vegfa
Gene Alias
Vegf, Vegf-a, Vegf120, Vegf164, Vegf188, Vpf
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000017463|OTTMUSP00000017464|OTTMUSP00000022243|vascular permeability factor
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