CD97 monoclonal antibody, clone 6D7E6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: CD97-hIgGFc transfected HEK293 cell lysates with CD97 monoclonal antibody, clone 6D7E6 (Cat # MAB21366).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human endometrium cancer with CD97 monoclonal antibody, clone 6D7E6 (Cat # MAB21366).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with CD97 monoclonal antibody, clone 6D7E6 (Cat # MAB21366).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with CD97 monoclonal antibody, clone 6D7E6 (Cat # MAB21366) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CD97.
Immunogen
Recombinant protein corresponding to amino acids 419-552 of human CD97.
Host
Mouse
Theoretical MW (kDa)
91.8
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: CD97-hIgGFc transfected HEK293 cell lysates with CD97 monoclonal antibody, clone 6D7E6 (Cat # MAB21366).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human endometrium cancer with CD97 monoclonal antibody, clone 6D7E6 (Cat # MAB21366).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with CD97 monoclonal antibody, clone 6D7E6 (Cat # MAB21366).Flow Cytometry
Flow cytometric analysis of HL-60 cells with CD97 monoclonal antibody, clone 6D7E6 (Cat # MAB21366) (Green). Red: Negative Control. -
Gene Info — CD97
Entrez GeneID
976Protein Accession#
P48960Gene Name
CD97
Gene Alias
TM7LN1
Gene Description
CD97 molecule
Omim ID
601211Gene Ontology
HyperlinkGene Summary
This gene is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The encoded product is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. The protein has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. Alternative splicing has been observed for this gene and three variants have been found. [provided by RefSeq
Other Designations
CD97 antigen|leukocyte antigen CD97|seven transmembrane helix receptor|seven-span transmembrane protein|seven-transmembrane, heterodimeric receptor associated with inflammation
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Interactome
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Disease
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