CD97 recombinant monoclonal antibody, clone 3A7
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More Files
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Immunohistochemistry
Immunohistochemical staining of human lymph node tissue with CD97 recombinant monoclonal antibody, clone 3A7 (Cat # RAB04219) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human spleen tissue with CD97 recombinant monoclonal antibody, clone 3A7 (Cat # RAB04219) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with CD97 recombinant monoclonal antibody, clone 3A7 (Cat # RAB04219) (diluated at 1:87.5). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD97.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD97.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:30-1:200)
Immunohistochemistry (1:50-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemical staining of human lymph node tissue with CD97 recombinant monoclonal antibody, clone 3A7 (Cat # RAB04219) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human spleen tissue with CD97 recombinant monoclonal antibody, clone 3A7 (Cat # RAB04219) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with CD97 recombinant monoclonal antibody, clone 3A7 (Cat # RAB04219) (diluated at 1:87.5). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD97
Entrez GeneID
976Protein Accession#
P48960Gene Name
CD97
Gene Alias
TM7LN1
Gene Description
CD97 molecule
Omim ID
601211Gene Ontology
HyperlinkGene Summary
This gene is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The encoded product is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. The protein has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. Alternative splicing has been observed for this gene and three variants have been found. [provided by RefSeq
Other Designations
CD97 antigen|leukocyte antigen CD97|seven transmembrane helix receptor|seven-span transmembrane protein|seven-transmembrane, heterodimeric receptor associated with inflammation
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Interactome
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Disease
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