Histone H3 monoclonal antibody, clone 1G1
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: HeLa cell, Lane 2: Raw 264.7 cell, Lane 3: mouse brain tissue and Lane 4: rat brain tissue.
Immunofluorescence
Immunofluorescence staining of HeLa cell line.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human histone H3.
Immunogen
Recombinant protein corresponding to full length human histone H3.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1:100-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:300)
Immunoprecipitation (1:200)
Western Blot (1:2000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HeLa cell, Lane 2: Raw 264.7 cell, Lane 3: mouse brain tissue and Lane 4: rat brain tissue.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Immunofluorescence staining of HeLa cell line.Immunoprecipitation
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Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P68431Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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Gene Info — HIST1H3D
Entrez GeneID
8351Protein Accession#
P68431Gene Name
HIST1H3D
Gene Alias
H3/b, H3FB
Gene Description
histone cluster 1, H3d
Omim ID
602811Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H3 histone family, member B|OTTHUMP00000016149|histone 1, H3d
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Gene Info — HIST1H3C
Entrez GeneID
8352Protein Accession#
P68431Gene Name
HIST1H3C
Gene Alias
H3.1, H3/c, H3FC
Gene Description
histone cluster 1, H3c
Omim ID
602812Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H3 histone family, member C|histone 1, H3c
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Gene Info — HIST1H3E
Entrez GeneID
8353Protein Accession#
P68431Gene Name
HIST1H3E
Gene Alias
H3.1, H3/d, H3FD
Gene Description
histone cluster 1, H3e
Omim ID
602813Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H3 histone family, member D|histone 1, H3e
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Gene Info — HIST1H3I
Entrez GeneID
8354Protein Accession#
P68431Gene Name
HIST1H3I
Gene Alias
H3.f, H3/f, H3FF
Gene Description
histone cluster 1, H3i
Omim ID
602814Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member F|OTTHUMP00000017803|histone 1, H3i
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Gene Info — HIST1H3G
Entrez GeneID
8355Protein Accession#
P68431Gene Name
HIST1H3G
Gene Alias
H3/h, H3FH
Gene Description
histone cluster 1, H3g
Omim ID
602815Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H3 histone family, member H|OTTHUMP00000016152|histone 1, H3g
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Gene Info — HIST1H3J
Entrez GeneID
8356Protein Accession#
P68431Gene Name
HIST1H3J
Gene Alias
H3/j, H3FJ
Gene Description
histone cluster 1, H3j
Omim ID
602817Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member J|OTTHUMP00000017804|histone 1, H3j
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Gene Info — HIST1H3H
Entrez GeneID
8357Protein Accession#
P68431Gene Name
HIST1H3H
Gene Alias
FLJ92264, H3/k, H3F1K, H3FK
Gene Description
histone cluster 1, H3h
Omim ID
602818Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member K|histone 1, H3h
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Gene Info — HIST1H3B
Entrez GeneID
8358Protein Accession#
P68431Gene Name
HIST1H3B
Gene Alias
H3/l, H3FL
Gene Description
histone cluster 1, H3b
Omim ID
602819Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member L|OTTHUMP00000016132|histone 1, H3b
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Gene Info — HIST1H3F
Entrez GeneID
8968Protein Accession#
P68431Gene Name
HIST1H3F
Gene Alias
H3/i, H3FI
Gene Description
histone cluster 1, H3f
Omim ID
602816Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member I|OTTHUMP00000016151|histone 1, H3f
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Interactome
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Pathway
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