Histone H3.1 recombinant monoclonal antibody, clone 27F2
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More Files
- More Functions
Western Blot
Western blot analysis of Jurkat whole cell lysate with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213).
Immunohistochemistry
Immunohistochemical staining of human colon cancer with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human glioma cancer with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213) (diluated at 1:93). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cells with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human Histone H3.1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human histone H3.1.
Theoretical MW (kDa)
Calculated MW: 15 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:30-1:200)
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Jurkat whole cell lysate with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213).Immunohistochemistry
Immunohistochemical staining of human colon cancer with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human glioma cancer with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213) (diluated at 1:93). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with Histone H3.1 recombinant monoclonal antibody, clone 27F2 (Cat # RAB04213) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P68431Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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Interactome
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Pathway
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