HIST1H3A recombinant monoclonal antibody, clone 30A7
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More Files
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Immunohistochemistry
Immunohistochemistry image of HIST1H3A recombinant monoclonal antibody, clone 30A7 diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of A549 Cells with HIST1H3A recombinant monoclonal antibody, clone 30A7 at 1:50, counter-stained with DAPI.
Flow Cytometry
Overlay Peak curve showing A549 cells stained with HIST1H3A recombinant monoclonal antibody, clone 30A7 (red line) at 1:100.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HIST1H3A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HIST1H3A.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemistry image of HIST1H3A recombinant monoclonal antibody, clone 30A7 diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of A549 Cells with HIST1H3A recombinant monoclonal antibody, clone 30A7 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing A549 cells stained with HIST1H3A recombinant monoclonal antibody, clone 30A7 (red line) at 1:100. -
Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P68431Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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Interactome
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Pathway
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