BCL2 monoclonal antibody, clone 100/D5
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of human skin using BCL2 monoclonal antibody, clone 100/D5 (Cat# MAB11293) at 2 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of non-Hodgkin's lymphoma with BCL2 monoclonal antibody, clone 100/D5 (Cat # MAB11293) at 1:500 using peroxidase-conjugate and DAB chromogen (Note: cell membrane and cytoplasmic staining).
Flow Cytometry
Flow cytometric analysis of Jurkat cells using BCL2 monoclonal antibody, clone 100/D5 (Cat # MAB11293) (red) and isotype control (green) at 0.5 ug/106 cells.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of BCL2.
Immunogen
A synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG-Cys) of human BCL2 (Pezzella et al, 1990).
Host
Mouse
Reactivity
Human, Monkey
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1-3 ug/ml)
Flow Cytometry (0.5 ug/l06 cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:250-1:1500)
Immunoprecipitation (1:10 - 1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of human skin using BCL2 monoclonal antibody, clone 100/D5 (Cat# MAB11293) at 2 ug/ml.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of non-Hodgkin's lymphoma with BCL2 monoclonal antibody, clone 100/D5 (Cat # MAB11293) at 1:500 using peroxidase-conjugate and DAB chromogen (Note: cell membrane and cytoplasmic staining).Flow Cytometry
Flow cytometric analysis of Jurkat cells using BCL2 monoclonal antibody, clone 100/D5 (Cat # MAB11293) (red) and isotype control (green) at 0.5 ug/106 cells. -
Gene Info — BCL2
Entrez GeneID
596Protein Accession#
NP_000648.2Gene Name
BCL2
Gene Alias
Bcl-2
Gene Description
B-cell CLL/lymphoma 2
Omim ID
151430Gene Ontology
HyperlinkGene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
Other Designations
B-cell lymphoma protein 2|OTTHUMP00000163680
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Interactome
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Pathway
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Disease
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