TNNI1 monoclonal antibody, clone AT36E7
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Mouse muscle extract (40 ug) by using TNNI1 monoclonal antibody, clone AT36E7 (Cat # MAB11204) (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 1:500. Lane 2: 1:1000. Lane 3: 1:5000.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant TNNI1.
Immunogen
Recombinant protein corresponding to amino acids 1-187 of human TNNI1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Mouse muscle extract (40 ug) by using TNNI1 monoclonal antibody, clone AT36E7 (Cat # MAB11204) (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 1:500. Lane 2: 1:1000. Lane 3: 1:5000.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TNNI1
Entrez GeneID
7135Protein Accession#
NP_003272Gene Name
TNNI1
Gene Alias
DKFZp451O223, SSTNI, TNN1
Gene Description
troponin I type 1 (skeletal, slow)
Omim ID
191042Gene Ontology
HyperlinkGene Summary
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq
Other Designations
OTTHUMP00000033877|OTTHUMP00000033910|slow-twitch skeletal troponin I|troponin I, skeletal, slow
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