HSP90AB1 monoclonal antibody, clone 5G4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) against Jurkat (1) , A-431 (2) , HeLa (3) , A-549 (4) , HEK293 (5) , K-562 (6) , NIH/3T3 (7) , PC-12 (8) and COS-7 (9) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues (upper) and brain tissues (bottom) using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) .
Flow Cytometry
Flow cytometric analysis of HeLa cells using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HSP90AB1.
Immunogen
Recombinant protein corresponding to human HSP90AB1.
Host
Mouse
Theoretical MW (kDa)
84
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) against Jurkat (1) , A-431 (2) , HeLa (3) , A-549 (4) , HEK293 (5) , K-562 (6) , NIH/3T3 (7) , PC-12 (8) and COS-7 (9) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues (upper) and brain tissues (bottom) using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) .Flow Cytometry
Flow cytometric analysis of HeLa cells using HSP90AB1 monoclonal antibody, clone 5G4 (Cat # MAB10686) (green) and negative control (purple). -
Gene Info — HSP90AB1
Entrez GeneID
3326Gene Name
HSP90AB1
Gene Alias
D6S182, FLJ26984, HSP90-BETA, HSP90B, HSPC2, HSPCB
Gene Description
heat shock protein 90kDa alpha (cytosolic), class B member 1
Omim ID
140572Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
OTTHUMP00000016517|OTTHUMP00000016518|OTTHUMP00000016519|OTTHUMP00000039869|heat shock 90kD protein 1, beta|heat shock 90kDa protein 1, beta|heat shock protein beta
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Interactome
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Pathway
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Disease
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