PPP1R8 monoclonal antibody (M05), clone 4B5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PPP1R8 expression in transfected 293T cell line by PPP1R8 monoclonal antibody (M05), clone 4B5.
Lane 1: PPP1R8 transfected lysate (Predicted MW: 38.5 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PPP1R8 transfected lysate using anti-PPP1R8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPP1R8 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPP1R8 is approximately 3ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PPP1R8.
Immunogen
PPP1R8 (NP_002704.1, 28 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PPP1R8 expression in transfected 293T cell line by PPP1R8 monoclonal antibody (M05), clone 4B5.
Lane 1: PPP1R8 transfected lysate (Predicted MW: 38.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PPP1R8 transfected lysate using anti-PPP1R8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPP1R8 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPP1R8 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — PPP1R8
Entrez GeneID
5511GeneBank Accession#
NM_002713Protein Accession#
NP_002704.1Gene Name
PPP1R8
Gene Alias
ARD-1, ARD1, NIPP-1, NIPP1, PRO2047
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 8
Omim ID
602636Gene Ontology
HyperlinkGene Summary
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq
Other Designations
OTTHUMP00000003847|OTTHUMP00000003848|OTTHUMP00000003849|OTTHUMP00000044938|RNase E|activator of RNA decay|nuclear inhibitor of protein phosphatase-1|nuclear subunit of PP-1|protein phosphatase 1 regulatory inhibitor subunit 8|protein phosphatase 1 regula
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