PPP1R8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PPP1R8 polyclonal antibody (Cat # PAB6447) staining (2 ug/mL) of NCI-H460 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PPP1R8 polyclonal antibody (Cat # PAB6447) (3.8 ug/mL) staining of paraffin embedded human thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPP1R8.
Immunogen
A synthetic peptide corresponding to C terminus of human PPP1R8.
Sequence
C-EAWPGKKPTPSLLI
Host
Goat
Theoretical MW (kDa)
13.3, 38.5, 22.7
Reactivity
Human
Specificity
This antibody is expected to recognize all three human isoforms of this protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-2 ug/mL)br>Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PPP1R8 polyclonal antibody (Cat # PAB6447) staining (2 ug/mL) of NCI-H460 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PPP1R8 polyclonal antibody (Cat # PAB6447) (3.8 ug/mL) staining of paraffin embedded human thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP1R8
Entrez GeneID
5511Protein Accession#
NP_002704;NP_054829;NP_612568Gene Name
PPP1R8
Gene Alias
ARD-1, ARD1, NIPP-1, NIPP1, PRO2047
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 8
Omim ID
602636Gene Ontology
HyperlinkGene Summary
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq
Other Designations
OTTHUMP00000003847|OTTHUMP00000003848|OTTHUMP00000003849|OTTHUMP00000044938|RNase E|activator of RNA decay|nuclear inhibitor of protein phosphatase-1|nuclear subunit of PP-1|protein phosphatase 1 regulatory inhibitor subunit 8|protein phosphatase 1 regula
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Interactome
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Publication Reference
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The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly.
Beullens M, Bollen M.
The Journal of Biological Chemistry 2002 May; 277(22):19855.
Application：IP, Recombinant protein.
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The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly.
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