E. coli is a well-established host that offers rapid expression, easy genetic manipulation, high yield, and low cost for a wide variety of recombinant protein production. Abnova provides custom E. coli protein expression services encompassing codon optimization, gene synthesis, vector construction, protein expression, protein purification, and validation. The purified protein is checked by SDS-PAGE, Western Blot, endotoxin detection, and bioactivity required by the customers. Our E. coli expression platform delivers the best quality proteins for your research.

Flowchart

 
 
 

Specification

E. coli Protein Expression Service Details

Expression vector

Polyhistidine tag at the N-terminus 

Timeline

Phase I

Cloning of your gene of interest into a vector with 6x His tag at the N-terminus

4 weeks

Phase II

50 mL, 100mL, 500mL, or 1L expression with E.coli expression platform 

4 weeks

Phase III

Protein purification using Ni-NTA affinity purification columns

Deliverables

• Purified protein     • SDS-PAGE report     • Western blotting     • CoA
 

Advantages
  • High quality guaranteed
  • Cost-effective
  • Rapid expression
  • High yield capacity
  • Easy scale-up
  • Ease of genome modification
  • Suitable for multiple species protein expression
 
 

Examples
Human GM-CSF Biological Activity
 

 
Measured by its ability to induce TF-1 cell proliferation. The specific activity of recombinant human GM-CSF is > 1 x 107 IU/mg. The ED50 for this effect is < 80 pg/mL.
 
Human TGFβ1 Biological Activity
 

 
Measured by its ability to induce proliferation in MCF-7 cells. The specific activity of recombinant human TGFβ1 is > 5 x 107 IU/mg. The ED50 for this effect is < 3.2 ng/mL.
 
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