Abnova provides a total solution for antibody pair development for quantitative measurement of your protein of interest. Leveraging our mouse monoclonal antibody manufacturing capability, and our new MaxPab® technology for the production of rabbit polyclonal antibody from full-length protein, Abnova allows you to focus on more important parts of your research instead of the arduous task of searching and testing for the matching antibody components. You may use our antibody pair in conventional sandwich ELISA or system applications.

General Working Progress
  • cDNA Sourcing and Cloning
  • Recombinant Protein Expression and Purification
  • Mouse and Rabbit Immunization
  • Screening Matched Mouse mAb and Rabbit pAb
  • Standard Curve Construction using Recombinant Protein
  • Validation on Mammalian Transfected Lysates for Native Protein Recognition
  • Delivery of Mouse Hybridoma and Rabbit pAb Serum

Advantages
  • High Specificity
  • High Signal to Background Ratio
  • Multiple Epitopes Target Detection
  • Amenable to S-ELISA and System Applications
   

Antibody Production

Antibody-Pair_flowchart1.jpg (147 KB)

Antibody Pair for Sandwich ELISA

Antibody-Pair_flowchart2.jpg (186 KB)
   

Colorimetric Detection

Paired Antibodies :
GCG Mouse Monoclonal Antibody (H00002641-M01)
GCG Mouse Monoclonal Antibody

 

antibody_pair1.png (78 KB)

Paired Antibodies :
AHSG Mouse Monoclonal Antibody (H00000197-M01)
AHSG Mouse Monoclonal Antibody

 

antibody_pair2.png (68 KB)
   

 
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