HSP90AB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with TNF-a (20 ng/mL, 30 min), using HSP90AB1 polyclonal antibody (Cat # PAB18096).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90AB1 polyclonal antibody (Cat # PAB18096).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with TNF-a (20 nM, 15 mins), using HSP90AB1 polyclonal antibody (Cat # PAB18096).
Peptide "+" means "peptide blocking".
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HSP90AB1.
Immunogen
A synthetic peptide corresponding to human HSP90AB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to HSP90AB1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with TNF-a (20 ng/mL, 30 min), using HSP90AB1 polyclonal antibody (Cat # PAB18096).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90AB1 polyclonal antibody (Cat # PAB18096).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with TNF-a (20 nM, 15 mins), using HSP90AB1 polyclonal antibody (Cat # PAB18096).
Peptide "+" means "peptide blocking".Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSP90AB1
Entrez GeneID
3326Protein Accession#
P08238Gene Name
HSP90AB1
Gene Alias
D6S182, FLJ26984, HSP90-BETA, HSP90B, HSPC2, HSPCB
Gene Description
heat shock protein 90kDa alpha (cytosolic), class B member 1
Omim ID
140572Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
OTTHUMP00000016517|OTTHUMP00000016518|OTTHUMP00000016519|OTTHUMP00000039869|heat shock 90kD protein 1, beta|heat shock 90kDa protein 1, beta|heat shock protein beta
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Interactomes
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Pathways
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Diseases
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Publication Reference
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A nonredundant human protein chip for antibody screening and serum profiling.
Lueking A, Possling A, Huber O, Beveridge A, Horn M, Eickhoff H, Schuchardt J, Lehrach H, Cahill DJ.
Molecular and Cellular Proteomics 2003 Dec; 2(12):1342.
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Domain mapping studies reveal that the M domain of hsp90 serves as a molecular scaffold to regulate Akt-dependent phosphorylation of endothelial nitric oxide synthase and NO release.
Fontana J, Fulton D, Chen Y, Fairchild TA, McCabe TJ, Fujita N, Tsuruo T, Sessa WC.
Circulation Research 2002 May; 90(8):866.
Application：IP, WB, Bovine, Bovine aortic endothelial cells.
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A nonredundant human protein chip for antibody screening and serum profiling.
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