AKT2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from A2780 (Lane 1), 293 (Lane 2) and LOX (Lane 3 and lane 4) using AKT2 polyclonal antibody (Cat # PAB18079).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AKT2 polyclonal antibody (Cat # PAB18079).
Peptide "+" means "with peptide blocking".
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT2.
Immunogen
A synthetic peptide corresponding to residues surrounding S474 of human AKT2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to AKT2.
Form
Liquid
Recommend Usage
Western Blot (1:500~1:1000)
Immunohistochemistry (1:50~1:100)
ELISA(1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A2780 (Lane 1), 293 (Lane 2) and LOX (Lane 3 and lane 4) using AKT2 polyclonal antibody (Cat # PAB18079).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AKT2 polyclonal antibody (Cat # PAB18079).
Peptide "+" means "with peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT2
Entrez GeneID
208Protein Accession#
P31751Gene Name
AKT2
Gene Alias
PKBB, PKBBETA, PRKBB, RAC-BETA
Gene Description
v-akt murine thymoma viral oncogene homolog 2
Gene Ontology
HyperlinkGene Summary
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Other Designations
Murine thymoma viral (v-akt) homolog-2|rac protein kinase beta
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Phosphatidylinositol-3-OH Kinase (PI3K)/AKT2, activated in breast cancer, regulates and is induced by estrogen receptor alpha (ERalpha) via interaction between ERalpha and PI3K.
Sun M, Paciga JE, Feldman RI, Yuan Z, Coppola D, Lu YY, Shelley SA, Nicosia SV, Cheng JQ.
Cancer Research 2001 Aug; 61(16):5985.
Application：IP, WB, Human, Human breast tumor specimens.
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Frequent activation of AKT2 and induction of apoptosis by inhibition of phosphoinositide-3-OH kinase/Akt pathway in human ovarian cancer.
Yuan ZQ, Sun M, Feldman RI, Wang G, Ma X, Jiang C, Coppola D, Nicosia SV, Cheng JQ.
Oncogene 2000 May; 19(19):2324.
Application：IHC, WB-Ti, Human, Ovarian carcinoma.
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Mitogenic activation, phosphorylation, and nuclear translocation of protein kinase Bbeta.
Meier R, Alessi DR, Cron P, Andjelkovic M, Hemmings BA.
The Journal of Biological Chemistry 1997 Nov; 272(48):30491.
Application：IP, WB-Tr, Human, HEK 293 cells.
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Phosphatidylinositol-3-OH Kinase (PI3K)/AKT2, activated in breast cancer, regulates and is induced by estrogen receptor alpha (ERalpha) via interaction between ERalpha and PI3K.
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