AKT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with Etoposide (25 uM, 24 hours), using AKT1 polyclonal antibody (Cat # PAB18072).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human ovary tissue using AKT1 polyclonal antibody (Cat # PAB18072).
Peptide "+" means "with peptide blocking".
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT1.
Immunogen
A synthetic peptide corresponding to residues surrounding S246 of human AKT1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to AKT1.
Form
Liquid
Recommend Usage
Western Blot (1:500~1:1000)
Immunohistochemistry (1:50~1:100)
ELISA(1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with Etoposide (25 uM, 24 hours), using AKT1 polyclonal antibody (Cat # PAB18072).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human ovary tissue using AKT1 polyclonal antibody (Cat # PAB18072).
Peptide "+" means "with peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactomes
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Pathways
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Diseases
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Publication Reference
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MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells.
Limesand KH, Schwertfeger KL, Anderson SM.
Molecular and Cellular Biology 2006 Dec; 26(23):8840.
Application：WB-Ti, Mouse, Salivary glands.
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Akt1 in the cardiovascular system: friend or foe?
O'Neill BT, Abel ED.
The Journal of Clinical Investigation 2005 Aug; 115(8):2059.
Application：WB, Human, Human mammalian cells .
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Protein kinase B alpha/Akt1 regulates placental development and fetal growth.
Yang ZZ, Tschopp O, Hemmings-Mieszczak M, Feng J, Brodbeck D, Perentes E, Hemmings BA.
The Journal of Biological Chemistry 2003 Aug; 278(34):32124.
Application：IHC-P, WB, Mouse, Placenta, Tissus.
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MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells.
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