ERBB2 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Her2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Her2 Antibody , hFc Tag with the EC50 of 0.20ug/ml determined by ELISA.
SEC-HPLC
The purity of Human Her2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Her2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specifications
Product Description
Human ERBB2 (P04626-1, Thr23-Thr652) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
Thr23-Thr652
Host
Human
Theoretical MW (kDa)
96.1 kDa
Due to gForm
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Her2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Her2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (8% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Her2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Her2 Antibody , hFc Tag with the EC50 of 0.20ug/ml determined by ELISA.Functional Study
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Gene Info — ERBB2
Entrez GeneID
2064Protein Accession#
P04626-1Gene Name
ERBB2
Gene Alias
CD340, HER-2, HER-2/neu, HER2, NEU, NGL, TKR1
Gene Description
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq
Other Designations
c-erb B2/neu protein|erbB-2|herstatin|neuroblastoma/glioblastoma derived oncogene homolog|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
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Interactomes
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Pathways
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Diseases
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