HSPA1A monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HSPA1A monoclonal antibody (Cat # MAB8734) at 1 : 500 dilution. Lane 1 : K-562 whole cell lysate 40 ug/Lane. Lane 2 : HeLa whole cell lysate 40 ug/Lane. Predicted band size : 70 KDa. Observed band size : 70 KDa.
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant HSPA1A.
Immunogen
Recombinant protein corresponding to C-terminus residues of human HSPA1A.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to HSPA1A.
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:100-1:2000)
ELISA (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HSPA1A monoclonal antibody (Cat # MAB8734) at 1 : 500 dilution. Lane 1 : K-562 whole cell lysate 40 ug/Lane. Lane 2 : HeLa whole cell lysate 40 ug/Lane. Predicted band size : 70 KDa. Observed band size : 70 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSPA1A
Entrez GeneID
3303GeneBank Accession#
NM_005345.5Gene Name
HSPA1A
Gene Alias
FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, HSPA1B
Gene Description
heat shock 70kDa protein 1A
Omim ID
140550Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
Other Designations
OTTHUMP00000029287|dnaK-type molecular chaperone HSP70-1|heat shock 70kD protein 1A|heat shock 70kDa protein 1B|heat shock-induced protein
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Interactomes
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Pathways
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Diseases
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