IL18 (Human) Recombinant Protein
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Functional Study
The ED50 for this effect is <6 ng/mL, measured by the induction of IFN gamma secretion in KG-1 cells.
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Specifications
Product Description
Human IL18 recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 18.2
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 0.05 EU/ug
Activity
The ED50 for this effect is < 6 ng/mL, measured by the induction of IFN gamma secretion in KG-1 cells. The specific activity of recombinant human IL-18 is > 1 x 107 IU/mg, which is calibrated against the human IL-18 WHO Reference Reagent (NIBSC code: 03/200).
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Regulatory Status
GMP grade
Storage Buffer
Lyophilized from PBS, pH 7.4.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
The ED50 for this effect is <6 ng/mL, measured by the induction of IFN gamma secretion in KG-1 cells.SDS-PAGE
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Gene Info — IL18
Entrez GeneID
3606Gene Name
IL18
Gene Alias
IGIF, IL-18, IL-1g, IL1F4, MGC12320
Gene Description
interleukin 18 (interferon-gamma-inducing factor)
Omim ID
600953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. [provided by RefSeq
Other Designations
IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18
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Interactomes
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Pathways
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Diseases
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