HAT1 monoclonal antibody
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant HAT1.
Immunogen
Recombinant protein corresponding to N-terminus residues of human HAT1.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to HAT1.
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:100-1:2000)
ELISA (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HAT1 monoclonal antibody (Cat # MAB8748) at 1 : 1000 dilution. Lane 1 : HeLa whole cell lysate 40 ug/Lane. Lane 2 : 293 whole cell lysate 40 ug/Lane. Lane 3 : NIH/3T3 whole cell lysate 40 ug/Lane. Predicted band size : 49 KDa. Observed band size : 49 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HAT1
Entrez GeneID
8520GeneBank Accession#
NM_003642.3Gene Name
HAT1
Gene Alias
KAT1
Gene Description
histone acetyltransferase 1
Omim ID
603053Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq
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