Mouse IgM monoclonal antibody, clone RM109

Catalog # MAB12772

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Size:100 ug
Price: USD $ 428.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of Mouse IgM monoclonal antibody, clone RM109 (Cat# MAB12772) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Mouse IgM monoclonal antibody, clone RM109 (Cat # MAB12772) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against mouse IgM.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against mouse IgM.

    Sequence

    N/A

    Reactivity

    Mouse

    Specificity

    This antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG.

    Form

    Liquid

    Purification

    Protein A affinity purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.005 - 0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5 - 2 ug/mL)
    Immunohistochemistry (0.5 - 2 ug/mL)
    Immunoprecipitation
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry

    Immunocytochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of Mouse IgM monoclonal antibody, clone RM109 (Cat# MAB12772) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Mouse IgM monoclonal antibody, clone RM109 (Cat # MAB12772) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — Igh-6

    Entrez GeneID

    16019

    Protein Accession#

    P01872

    Gene Name

    Igh-6

    Gene Alias

    AI326478, IGHM, Igh-M, Igh6, Igm, MGC107036, MGC107507, muH

    Gene Description

    immunoglobulin heavy chain 6 (heavy chain of IgM)

    Gene Ontology

    Hyperlink

    Other Designations

    -

Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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