Mouse IgG3 monoclonal antibody, clone RM218

Catalog # MAB12770

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Quantity

Size:100 ug
Price: USD $ 428.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Mouse IgG3 monoclonal antibody, clone RM218 (Cat # MAB12770) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both mouse IgG3 (kappa) and IgG3 (lambda); No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of Mouse IgG3 monoclonal antibody, clone RM218 (Cat# MAB12770) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against mouse IgG3.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against mouse IgG3.

    Sequence

    N/A

    Reactivity

    Mouse

    Specificity

    This antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.

    Form

    Liquid

    Purification

    Protein A affinity purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.005 - 0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5 - 2 ug/mL)
    Immunohistochemistry (0.5 - 2 ug/mL)
    Immunoprecipitation
    Western Blot (0.1 - 0.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunohistochemistry

    Immunocytochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Mouse IgG3 monoclonal antibody, clone RM218 (Cat # MAB12770) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both mouse IgG3 (kappa) and IgG3 (lambda); No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Enzyme-linked Immunoabsorbent Assay

    A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of Mouse IgG3 monoclonal antibody, clone RM218 (Cat# MAB12770) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — AI324046

    Entrez GeneID

    380795

    Protein Accession#

    P03987

    Gene Name

    AI324046

    Gene Alias

    IGHG3, IgG3, MGC68300

    Gene Description

    expressed sequence AI324046

    Gene Ontology

    Hyperlink

    Other Designations

    -

Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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