EGFR recombinant monoclonal antibody, clone 9F10
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EGFR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human EGFR.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemistry image of EGFR recombinant monoclonal antibody, clone 9F10 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of A549 Cells with EGFR recombinant monoclonal antibody, clone 9F10 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing Jurkat cells stained with EGFR recombinant monoclonal antibody, clone 9F10 (red line) at 1:50. -
Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
P00533Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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