EGFR recombinant monoclonal antibody, clone S3 12D
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EGFR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human EGFR.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of NIH3T3 cells with EGFR recombinant monoclonal antibody, clone S3 12D (Cat # RAB03428).
Immunofluorescence analysis of unpermearaformaldehyde NIH3T3 cells fixed on Shi-fix™ coverslips stained with the chimeric rsion of RAB03428 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with unimmunised r followed by staining with Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of mouse splenocytes with EGFR recombinant monoclonal antibody, clone S3 12D (Cat # RAB03428).
Mouse splenocytes were stained with unimmunized r antibody (black line) or the rmeric version of RAB03428 (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing the bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/mL and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — EGFR
Entrez GeneID
1956Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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