EGFR recombinant monoclonal antibody, clone 528
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More Files
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Immunofluorescence
Immunofluorescenct staining of Hela cells with EGFR recombinant monoclonal antibody, clone 528 (Cat # RAB03426).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells permeabilized with 0.15% Triton stained with the chimeric r version of RAB03426 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells with EGFR recombinant monoclonal antibody, clone 528 (Cat # RAB03426).
HeLa cells were stained with unimmunized r antibody (black line) or the rmeric version of RAB03426 (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing the bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/mL and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EGFR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against partially purified EGFR from A431 cells.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Neutralization
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Immunofluorescenct staining of Hela cells with EGFR recombinant monoclonal antibody, clone 528 (Cat # RAB03426).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells permeabilized with 0.15% Triton stained with the chimeric r version of RAB03426 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of HeLa cells with EGFR recombinant monoclonal antibody, clone 528 (Cat # RAB03426).
HeLa cells were stained with unimmunized r antibody (black line) or the rmeric version of RAB03426 (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing the bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/mL and cells analyzed using a FACSCanto flow-cytometer.Neutralization
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Gene Info — EGFR
Entrez GeneID
1956Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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