LMNB1 recombinant monoclonal antibody, clone R05-8D6
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: rat brain, Lane 3: C6, Lane 4: 3T3 and Lane 5: Hela lysates with LMNB1 recombinant monoclonal antibody, clone R05-8D6 (Cat # RAB02143).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer with LMNB1 recombinant monoclonal antibody, clone R05-8D6 (Cat # RAB02143). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LMNB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human LMNB1.
Theoretical MW (kDa)
Calculated MW: 66 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunoprecipitation(1:20)
Western Blot (1:5000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: rat brain, Lane 3: C6, Lane 4: 3T3 and Lane 5: Hela lysates with LMNB1 recombinant monoclonal antibody, clone R05-8D6 (Cat # RAB02143).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer with LMNB1 recombinant monoclonal antibody, clone R05-8D6 (Cat # RAB02143). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — LMNB1
Entrez GeneID
4001Protein Accession#
P20700Gene Name
LMNB1
Gene Alias
ADLD, LMN, LMN2, LMNB, MGC111419
Gene Description
lamin B1
Gene Ontology
HyperlinkGene Summary
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq
Other Designations
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Interactome
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Disease
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