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Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed A431 cells stained with the chimeric rabbit IgG version of 2F8 (Zalutumumab; HuMax-EGFR) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cellular junction staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01677-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.

Flow Cytometry

Flow-cytometry were stained with anti-unknown specificity antibody (black line) or the rabbit IgG version of 2F8 (Zalutumumab; HuMax-EGFR) (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Entrez GeneID

1956

Protein Accession#

P00533

Gene Name

EGFR

Gene Alias

ERBB, ERBB1, HER1, PIG61, mENA

Gene Description

epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Omim ID

131550 211980

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq

Other Designations

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor